EBTT 2021
Archived edition · Hybrid

EBTT 2021

Electroporation-Based Technologies and Treatments — International Scientific Workshop & Postgraduate Course

14–20 November 2021Ljubljana, Slovenia (on-site & online)5 ECTS · 34 CME

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

The postgraduate course was intended both for novices and experts in electroporation, including PhD students, researchers and users. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought — each participant submitting an abstract also gave a short oral presentation of their work. The course was accredited by the Medical Chamber of Slovenia, so participants received 34 CME credits.

The 2021 edition was held as a hybrid event: lectures and practical work were offered on-site in Ljubljana as well as through interactive online sessions for remote participants. Attendance was subject to Slovenia's COVID-19 "recovered / vaccinated / tested" (RVT) rule, checked at entry, with protective face masks mandatory.

Organised by the University of Ljubljana and the Institute of Oncology, Ljubljana. Read all about EBTT in an article published by MediaResearch Ltd, 2012.

Course content

Preliminary programme & topics

Electrical properties of cells & tissues

Resting & induced transmembrane voltage; dielectric properties of tissues; temperature & conductivity changes.

Physical chemistry of electroporation

Electrochemical membrane thermodynamics; molecular dynamics simulations.

Electroporation in vitro & in vivo

Protocols for cells in vitro and in vivo.

Devices & electrodes

Devices & electrodes for in vitro/in vivo work; tissue models of electroporation and their validation.

Electrochemotherapy of tumours

Basic mechanisms; preclinical studies; clinical use; current developments & future perspectives.

Gene transfection

Gene transfection in vitro/in vivo; electroporation for gene therapy and DNA vaccines.

Highlights of 2021

Faculty

Directors & lecturers

Co-DirectorDamijan Miklavčič
Co-DirectorLluis M. Mir

Faculty members & lecturers

  • Damijan MiklavčičUniversity of Ljubljana, Slovenia
  • Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
  • Marie-Pierre RolsCNRS & IPBS, Toulouse, France
  • Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
  • Mounir TarekUniversity of Lorraine, Nancy, France
  • P. Thomas VernierOld Dominion University, Norfolk, Virginia, USA
  • Julie GehlUniversity of Copenhagen, Denmark

Invited lecturers

  • Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
  • Oleksandr GryshkovLeibniz University Hannover, Garbsen, Germany
  • Richard HellerUniversity of South Florida, Dept. of Medical Engineering, Tampa, Florida, USA
  • Iris HaberkornETH Zurich, Switzerland
  • Erika KisMedical University of Szeged, Dept. of Dermatology, Hungary
  • Tadej KotnikUniversity of Ljubljana, Slovenia
  • Rodney P. O'ConnorÉcole des Mines de Saint-Étienne, Microelectronics Center of Provence, Gardanne, France
  • Lea RemsUniversity of Ljubljana, Slovenia
  • Christian ZemlinWashington University, School of Medicine in St. Louis, USA

Founding Faculty members

Ambassadors of the EBTT

Organising committee

Programme & schedule

Schedule downloads

PDFFinal Schedule

Full programme (last update 12.11.2021)

 PDFShort Presentations Programme

Participants' short oral presentations (last update 10.11.2021)

 PDFLaboratory Practical Work Schedule

On-site laboratory sessions

 PDFOnline Practical Work Schedule

Online laboratory sessions

Hands-on

Practical work & exercises

Due to the hybrid nature of the 2021 edition, practical work was offered as on-site sessions as well as interactive online training sessions for those participating remotely. Exercise sheets (PDF) as distributed to participants. The mode column notes whether each exercise was available on-site, online, or both.

Laboratory exercises

L1The influence of Mg2+ ions on gene electrotransfer efficiencyon-site · onlinePDF ↓
L2Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AMon-sitePDF ↓
L3Visualisation of local ablation zone distribution between two needle electrodeson-site · onlinePDF ↓
L4Effect of short high-frequency bipolar pulses on plasma membrane permeabilizationon-sitePDF ↓
L5Electrotransformation of Escherichia coli with plasmid DNAon-sitePDF ↓
L6Electroporation of planar lipid bilayerson-site · onlinePDF ↓
L7E. coli inactivation by pulsed electric fields in a continuous flow systemon-site · onlinePDF ↓
L8Analysis of electric field orientations on gene electrotransfer — visualization at the membrane levelon-sitePDF ↓
L9Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experimentson-sitePDF ↓
L10Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomographyon-site · onlinePDF ↓
L11Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPSonlinePDF ↓
L12Analysis of electric field orientations on gene electrotransfer efficiencyonlinePDF ↓

Computer modelling

C1Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage & electrode positionon-site · onlinePDF ↓
C2Numerical modelling of thermal effects during irreversible electroporation treatmentson-site · onlinePDF ↓
C3Molecular dynamics simulations of membrane electroporationon-site · onlinePDF ↓

Hardware development & measurement

H1Measurement of electroporation pulses with oscilloscope, and voltage and current probeson-site · onlinePDF ↓
H2Development of pulsed power generators for electroporationon-site · onlinePDF ↓

E-learning

E1Electroporation of cells and tissues — interactive e-learningon-site · onlinePDF ↓

Laboratory safety

S1Laboratory safetyPDF ↓
S2Electroporation hardware safetyPDF ↓

Abstracts & publication

Abstract submission

Participants submitted an abstract as PDF and MS Word file. For abstract writing the enclosed MS Word template was used.

PDFAbstract Submission Form PDFInstructions for Abstract Submission DOCAbstract Template

Getting here

Venue & important locations

EBTT 2021 was held at the University of Ljubljana, Faculty of Electrical Engineering, 14–20 November 2021. Floorplan of the Faculty of Electrical Engineering (PDF)

Proceedings

Electronic proceedings

EBTT 2021 proceedings cover

Electronic proceedings — all abstracts and course material of EBTT 2021.

Download proceedings (PDF)

Support

Supported by

Donau Lab Ljubljana Farapulse IGEA Iskra Medical Jafral Kobis LEROY Biotech Mediline Medtronic Omega Pulse Biosciences ISEBTT Bioelectrochemical Society

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456

Accommodation & travel arrangements

Congress Agency ANTANA PCO
Mrs. Nina Bernard
nina.bernard@antana-pco.com
tel +386 41 229 222

The EBTT series

Previous & other editions

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