November 14-20, 2021

ONLINE Event, Ljubljana, Slovenia

Electroporation-Based Technologies and Treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

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Preliminary programme:

Electrical properties of cells and tissues in electric field

  • Resting and induced transmembrane voltage
  • Dielectric properties of biological tissues
  • Temperature and conductivity changes in tissues
  • Physical chemistry of membrane electroporation

  • Electrochemical membrane thermodynamics
  • Molecular dynamics simulations
  • Electroporation in vitro and in vivo - protocols

  • Electroporation of cells in vitro
  • Electroporation of cells in vivo
  • Development of devices and electrodes

  • Devices for in vitro and in vivo experimental work
  • Electrodes for in vitro and in vivo
  • Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
  • Electrochemotherapy of tumors

  • Basic mechanisms of electrochemotherapy
  • Preclinical studies
  • Clinical use of electrochemotherapy
  • Current developments and future perspectives
  • Application of electroporation in gene transfection

  • Gene transfection in vitro
  • Gene transfection in vivo
  • Electroporation for gene therapy
  • Electroporation for DNA vaccine
  • Highlights of 2021:

  • Prophylactic and Therapeutic Applications of  Gene Electrotransfer, Richard Heller
  • Calcium electroporation, Stine Krog Frandsen
  • Wound healing, Ann Rajnicek
  • PEF in food treatment and microalgae, Alexander Mathys
  • The Biophysics of Cell Membrane Electroporation, Lea Rems
  • Cardiac ablation and defibrillation with pulsed electric fields, Christian Zemlin
  • Laboratory and practical work

  • The influence of Mg2+ ions on gene electotransfer efficiency
  • Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
  • Electropermeabilization detection with propidium iodide in plated cells
  • Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
  • Electrotransformation of Escherichia coli with plasmid DNA
  • E. coli inactivation in a continuous flow system
  • Electroporation of planar lipid bilayers
  • Analysis of electric field orentations on gene electrotransfer visualization at the membrane level
  • Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
  • Numerical Modeling of Thermal Effects during Irreversible Electroporation Treatments
  • Molecular dynamics simulations of membrane electroporation
  • Electroporation of cells and tissues - interactive e-learning
  • Measurement of electroporation pules with osciloscope; voltage and current probes
  • Development of pulse power generators for electroporation